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Stress response capacity (Fv/Fm at 690 nm and F690/F735 at F max ) of untransformed hybrid poplar, Populus × canescens (P. tremula × P. alba), and two transgenic lines overexpressing γ-ECS (γ-glutamylcysteine synthetase) either in the cytosol (cyt-ECS) or in the chloroplast (chl-ECS) was studied in response to the herbicide paraquat (4.0 × 10 -9  to 4.0 × 10 -6  m) for 21 days. Significant differences at sublethal (4.0 × 10 -7  m) and bleaching (4.0 × 10 -6  m) concentrations of paraquat were observed with about a two-fold and eight-fold decrease in the photosynthetic activity (Fv/Fm at 690 nm and F690/F735 at F max ), respectively. None of the gshI transgenic lines (cyt-ECS, chl-ECS) with elevated GSH content exhibited significant tolerance to paraquat.

RT-PCR Analysis and Stress Response Capacity of Transgenic gshI-Poplar Clones (Populus × canescens) in Response to Paraquat Exposure