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In cell suspension cultures of Taxus chinensis, 40 mg/l fungal elicitor from Aspergillus niger and 20 μm HgCl 2  elicited 5.7 and 3.6 mg/l taxol, which was a 9-fold and 5- fold increase vs. compared with the control, respectively. The fungal elicitor induced hydrogen peroxide (H 2 O 2 ) accumulation but HgCl 2  did not, indicating that H 2 O 2  was not necessary for enhancement of taxol induced by elicitor. Compared with the treatment with fungal elicitor alone, exogenous catalase, ascorbic acid, diphenylene iodonium and superoxide dismutase induced a 0.45, 0.4, 0.7 and 1.4-fold H 2 O 2 , but elicited taxol production, which was 0.98, 1.2, 1.1 and 0.9-fold, respectively, vs. non-treated cells. Elicitor-induced taxol production was not accorded with the amount of H 2 O 2  production.

Hydrogen Peroxide from the Oxidative Burst is Not Involved in the Induction of Taxol Biosynthesis in Taxus chinensis Cells