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A collection of Eupatorium semialatum from Guatemala was investigated for the occurrence of sesquiterpene lactones. In addition to the sesquiterpene cinnamoyloxyoplopanone (5), lactones of the eudesmanolide type were found and identified by NMR as derivatives of 8 β-hydroxyreynosin and 8 β-hydroxybalchanin, esterified with 2-substituted acrylic acids. The acid moieties were determined to be the enantiomeric (R)- and (S)-(2-hydroxy-1-methoxyethyl) acrylic acid, in 1, 2 and 4 respectively, and (1-hydroxy-ethyl) acrylic acid in 3.

Eudesmanolides and Further Terpenes from the Leaves of Eupatorium semialatum