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A study is reported on the modification of the B880-RC assembly of Chromatium minutissimum during octyl-β-D -glucopyranoside/dodecyl-β-D -maltoside/Deriphat polyacrylamide gel electrophoresis followed by electroelution with dodecyl-β-D-maltoside and high performance liquid chromatography with octyl-β-D-glucopyranoside according to the method developed by Kerfeld et al. (Biochim. Biophys. Acta 1185, 193-202 [1994a]) for isolation of the B820 subcomplexes of Chromatium purpuratum. The B880-RC assembly of Chromatium minutissimum isolated by electrophoresis was contaminated by the B 800-850 complex. It was further separated into four components, three of which were in agreement with the cited work: (i) colorless contaminations, (ii) the B880-RC assembly, (iii) the B 800-850 complex. In contrast with Kerfeld et al. (1994a), the fourth band was a band of free pigments (Bchl or Bchl-t-carotenoids) which had the same molecular mass as the B820 subcomplex of Chromatium purpuratum.

Does the B820 Subcomplex of the B880 Complex Retain Carotenoids?