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Triazine-resistant chloroplasts of the Canadian spring oilseed rape variety OAC Triton were transferred into four German winter oilseed rape lines and two cultivars of double-low quality by means of protoplast fusion. X-irradiation has been used to reduce the amount of nuclear D N A of the spring type cultivar and to promote cybrid formation. RFLP-analysis showed that some regenerants and their progeny carried both types of chloroplasts. In some instances regenerants and progeny containing mixtures of both chloroplasts not kept under selective conditions lost their triazine-resistant chloroplasts completely during further plant growth. Preliminary results of greenhouse and field experiments indicate that volunteer plants can be eliminated by application of 150-300 g/ha metribuzin (Sencor R , Bayer AG) in a stand of triazine-resistant oilseed rape of double-low quality.

Segregation of Triazine-Resistant Chloroplasts after Introduction into Winter Type Oilseed Rape (Brassica napus L.) by Protoplast Fusion