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The range of inorganic acid normalities for maximum color formation of the phosphomolybdenum- blue complex (under heating) increases by elevating the ammonium molybdate concentration, and at a ratio of molybdate molarity/acid normality equal to 10, there is maximum color development at any acid normality in the range 1-4 ɴ with either HClO 4  or H 2 SO 4  (or their mixtures).

Optimization of Conditions for Accurate Phosphonate and Total Phosphorus Assay on Lipid Samples, in Conjunction with Thin-Layer Chromatography