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The ratios of the scoparone O-demethylation products scopoletin to isoscopoletin were deter mined for reconstituted complexes of NADPH-P-450 reductase and each of four P-450 isozymes in a 2:1 molar ratio with a 1:1 mixture of [7-O-methyl- 14 C]-and [6-O-methyl- 14 C]-scoparone as substrate. The two phenobarbital inducible forms P-450 PB-B  and P-450 PB-D  have a 1:0.8 ± 0.05 scopoletin to isoscopoletin ratio, and the two β-naphthoflavone inducible forms P-450 βNF-B  and P-450 βNF/ISF-G  have ratios of 1:4.4 ± 0.1 and 1:3.8 ± 0.1, respectively. The scoparone-O-demethylation activities of the reconstituted preformed complexes of the four P-450 isozymes are given.

Regioselective O-Demethylation of Scoparone: Differentiation between Rat Liver Cytochrome P-450 Isozymes