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The maximal binding of antibodies against the subunits of the coupling factor o f photo­ phosphorylation onto the ultrasonic sediment and to stroma-freed chloroplasts of Antirrhinum majus was determined. Different surfaces o f the thylakoid membrane are accessible to antibodies in both chloroplast preparations. Stroma-freed chloroplasts bind antibodies only at the outer surface, which is directed towards the stroma in intact chloroplasts. In the ultrasonic sediment, which is the product of ultrasonication and centrifugation, as was demonstrated by electronmicroscopy, the major part of the surface directed towards the inside of the thylakoids is accessible.

zeitschrift für naturforschung c

Binding of Antibodies onto the Thylakoid Membrane VII. Localization of Coupling Factor of Photophosphorylation in the Lamellar System of Chloroplasts from Antirrhinum majus