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Enzymological studies at high hydrostatic pressure generally involve temperature, pH and pressure as variables, owing to the effect of adiabatic compression and the ionization volume of the buffer system. In the case of NAD dependent oxidoreductases this implies that the extinction coefficient of the coenzyme may be affected by p, T and pH, apart from the spectral change accompanying the redox reaction.

Pressure, Temperature and pH Dependence of the Absorption Spectrum of Reduced Nicotinamide Adenine Dinucleotide