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From solid phase peptide synthetic approaches of mMIF(93-115) the C-terminal sequence turned out to be a typical „difficult sequence“ peptide. Various stepwise syntheses were performed with different resins and coupling reagents. The Peptide was synthesized in 90% yield by solid phase fragment condensation of protected mMIF(93-108) to mMIF(109-115) ‘2-Chlorotrityl’ resin bound.

zeitschrift für naturforschung b

Zur Festphasenpeptidsynthese des Makrophagen-migrationsinhibierenden Faktors der Maus (mMIF): Ein Vergleich verschiedener Syntheseansätze zur Darstellung der schwierigen C-terminalen Sequenz mMIF(93-115)/ Solid Phase Peptide Synthetic Approaches to Mouse Macrophage Migration Inhibitory Factor (mMIF): Comparison of Different Strategies Producing the C-Terminal Difficult Sequence Peptide mMIF (93-115)