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The isolation and chromatographic separation of “Amylocyanin”, the blue pigment mixture of Streptomyces coelicolor is described. The main component is 5-(hydroxy-3-β-D-ribopyranosyl-pyrid-2-on-5-yl)-imino-3-β-D-ribopyranosyl-2,6-pyridindione (1), as is prooved by degradation reactions, model compounds and spectroscopic data. The nine minor components are α- and β-D-ribopyranosyl- and α- and β-ribofuranosyl derivatives of 1. Degradation with acetic acid/phenylhydrazine yields the isomere 3-α- and 3-β-D-ribopyranosyl- and 3-α- and 3-β-D-ribofuranosyl-5-phenylhydrazono-2,6-pyridindiones 2, 8, 9 and 10; ozonolysis of 1 liberates D-ribose.

zeitschrift für naturforschung b

Isolierung, Auftrennung und Strukturaufklärung des blauen Bakterienpigments „Amylocyanin" aus Streptomyces coelicolor Müller / Isolation, Separation and Structure of the Blue Pigment „Amylocyanin“ from Streptomyces coelicolor Müller