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Extracts of Chlorobium thiosulfatophilum and of Rhodospirillum rubrum have been tested for the presence of citrate lyase under various conditions. This enzyme could not be detected. It is, therefore, concluded that a complete reductive carboxylic acid cycle does not occur in these microorganisms.

Lack of Citrate Lyase — the Key Enzyme of the Reductive Carboxylic Acid Cycle — in Chlorobium thiosulfatophilum and Rhodospirillum rubrum