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Ehrlich ascites tumor cells adapted to serial cultivation in vitro in media with increased NaCl content (“high-salt”-tolerant cells) exhibit — in comparison with cells cultivated either in isotonic media or intraperitoneally in mice (in vivo) — an increased rate of cellular oxygen consumption. It is suggested that this increased respiratory rate of “high-salt”-tolerant cells reflects enhanced energy requirements associated with processes of active ion transport; and that the elevated environmental NaCl concentration may have “induced” a “step up” of the respiratory metabolism of these tumor cells.

Increased Oxygen Consumption of Ehrlich Ascites Tumor Cells Following Serial Cultivation in Media with Increased (Hypertonic) NaCl Content