English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Komagataella phaffii  is an important cell factory for recombinant protein production under methanol induction. Tracking of recombinant protein expression by single  K. phaffii  cell is a hot topic to identify the variation of expression level in submerge cultivation system. In this study, a platform system harnessing  Aspergillus niger  pellets for  K. phaffii  cell immobilization in fabricated glass plate was developed to measure the green fluorescent protein (GFP) expression of single cell using fluorescence microscope. This system was optimized through pellets preparation,  K. phaffii  cell absorption, inducer comparison, methanol concentration, flow velocity of medium, and obtaining a successful platform to track GFP expression of single  K. phaffii  cell. This system provided an on-line analytical technology to discover the heterogeneous capability of various  K. phaffii  cells.

Real-time monitoring of recombinant GFP expression in single-cell Komagataella phaffii through filamentous pelletization technology