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Quantitative measurements of labor stress exerted at 15,000 rpm at 4 °C. The plasma was removed, on the fetus are thought to be difficult (SUREAU frozen and stored at 20 °C för fürther hormonal [14]). Although the fetal adrenal and sympathetic assays. Another 5 ml each of blood was withdrawn nervous Systems are estimated to play a major role by a heparinized syringe from maternal peripheral in regulating circulatory and metabolic functions vein, umbilical cordvein and artery. Determinations under normal and abnormal circumstances during of blood Po2, Pcp2, pH and base deficit were made labor, previous studies have failed to document by a Radiometer bloodgas analyzer. Enzymatic consistent changes in fetal adrenergic activities at Analyses of glucose, lactate, pyruvate, glycerol, the perinatal period. In pursuit of better indexes and so on were made by the methods described by which might closely relate to the stress of labor, BERGMEYER [1]. Finally, 3 ml of blood was plasma concentrations of nor-adrenalin, adrenalin, collected in the iced centrifuge tube, and centricortisol, aldosterone, äs well äs, immunoreactive fuged at 4 °C. Plasma concentrations of free fatty (I.R.) insulin were measured in both maternal and acid (FFA) and inorganic phosphorous (Pj) were umbilical cord venous blood, and correlated with determined by WAKO-JUNYAKU Kit and FISKEquantitative cardiotocographic and mechanocardioSUBBAROW method, respectively. graphic values, Apgar scores, and umbilical cord The method for measuring plasma noradrenalin arterial blood biochemical piofilings in high-risk and adrenalin concentrations was the trihydroxypregnancies. indole method using K3Fe(CN)6 in the high-performance liquid chromatography, described by „ . . , , A, , MATSUDA et al [91. Catecholamine HPLC-Auto l Matenals and methods . · ^ j i ^ ^ *, analyzer consisted of a piston-type pump (KyowaDuring a year and a half period from September seimitsu KSU-P45H), a glass Zipax column 1976, through February 1978, blood samples of (DuPont, 2 mm ± 70 cm), an auto-sample injector 10 ml each were collected from maternal peripheral (HISSCO), an effluent exchanger, a pröportioning and umbilical cord veins immediately following pump (Technicon AIII), a fluorometer (Turner deliveries in a total of 70 high-risk pregnancies at 111), and a process sequencer (Tokai B-90XA). the Fukuoka University Hospital. The blood was A trace dose of epinine was added äs an internal collected in an iced centrifuge tube containing Standard for correction of recovery rate. The senEDTA-2Na, and centrifuged at once for20minutes sitivity of measuring method for nor-adrenalin

journal of perinatal medicine

Plasma-nor-adrenalin and adrenalin concentrations in feto-maternal blood: Their relations to feto-maternal endocrine levels, cardiotocographic and mechanocardiographic values, and umbilical arterial blood biochemical profilings