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The article presents the main results of a field research carried out between 2012 and 2014. It is a comparison case study of vegetation diversity of pools and dead-end branches of the Orlice River in Eastern Bohemia, Czech Republic. The actual state of wetland vegetation is being compared to data from literature that is several decades old. Botanical survey was conducted in the study area located between the town of Hradec Králové and parishes of Malšovice, Malšova Lhota a Svinary. The research confirmed that dead-end river branches and oxbow lakes form potential refugia of biodiversity of vascular plants in the landscape context of the river area despite the fact that the River Orlice was fully regulated in the urban area of Hradec Králové and the surrounding parishes in the past. Nevertheless, the river area is of a natural character there and belongs to the Nature Park of Orlice since 1996. The local ecosystems are also a part of the important locality of the River Orlice and Elbe. The total of 449 taxons of vascular plants (on the level of species and sub-species) and species rich associations of aquatic and wetland plants were recorded in the study area. Conservation of the water regime in the fluvial landscape and suitable regulation interventions against some spreading invasive species are crucial for the continuing existence of the precious species composition. For this reason the article also includes some management steps

Floodplain of the Orlice River (Eastern Bohemia, Czech Republic): The Natural Centre of Biodiversity