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To improve the extraction efficiency and quality, ultrasound-assisted solvent enzymatic extraction (UASEE) method was applied to extract oil from  Elaeagnus mollis  Diels. The soxhlet extraction (SE) and cold pressing (CP) were carried out to be compared with UASEE. The optimal UASEE conditions were liquid–solid ratio of 11.6 mL/g, enzyme amount of 1.1%, and ultrasound power of 583 W, which gave by Plackett–Burman and Box–Behnken design. Under optimum conditions, the oil yield of 43.35 ± 0.26% was reached, which was similar to that of SE (43.02 ± 0.77%). The oil obtained by UASEE is more desirable than that of SE, but similar with CP, especially on unsaturated fatty acids content and total tocopherol content. Overall, UASEE is a potential alternative to traditional methods for the efficient extraction of  E. mollis  oil for its higher oil yield and better quality.

Evaluation of yield and quality properties of Elaeagnus mollis oil produced by ultrasound-assisted solvent enzymatic extraction