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In the present work, an endemic species  Prangos heyniae  collected in four locations from Turkey was subjected to hydrodistillation in Clevenger type apparatus to obtain the essential oils (EO1-4). The gas-chromatography/mass spectrometry (GC/MS) and gas-chromatography-flame ionization detector (GC/FID) analyses showed that the EOs were rich in sesquiterpenes, germacrene D (10.3-12.1%), β-bisabolene (14.4%), kessane (26.9%), germacrene B (8.2%), elemol (3.4-46.9%), β-bisabolenal (1.4-70.7%), β-bisabolenol (8.4%) and an eudesmane type sesquiterpene ( 1 ) (16.1%) with [M+218]. This unidentified compound ( 1 ) was isolated in a rapid one-step manner with &gt;95.0% purity using Preparative Capillary Gas Chromatography (PCGC) with an HP Innowax column connected to a Preparative Fraction Collector (PFC) system. Structure determination was accomplished from 1D- and 2D-NMR spectroscopic data which determined a new eudesmane type sesquiterpene, 3,7(11)-eudesmadien-2-one ( 1 ). Using a biting deterrent bioassay, the mean proportion not biting (PNB) values of the  P .  heyniae  EO1-4 were 0.88 for EO1 and 0.80 for EO2 which were similar to the positive control DEET ( N , N -diethyl-3-methylbenzamide). The EO3 and EO4 had lower PNB values of 0.64 and 0.44, respectively.  P .  heyniae  EO1-4 showed good larvicidal activity at 125 and 62.5 ppm whereas EO1-3 were slightly less effective at the dose of 31.25 ppm and EO4 was not active at 31.25 ppm against 1 st  instar  Aedes aegypti .

open chemistry

Isolation of eudesmane type sesquiterpene ketone from  Prangos heyniae  H.Duman &amp; M.F.Watson essential oil and mosquitocidal activity of the essential oils