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The mechanism of action of non-steroidal anti-inflammatory drugs which are used in high doses in chronic inflammatory conditions is not clearly understood. Their known protein-stabilizing properties could play a significant role. The inhibition of cyclooxygenase may not be essential for their anti-rheumatic activity, since compounds with strong anti-denaturant properties and devoid of anti-inflammatory activity were shown to be effective in an experimental model of rheumatoid arthritis. Hence, to develop new anti-rheumatic drugs it is essential that a simple  in vitro  method to evaluate the anti-denaturant activity of endogenous and exogenous compounds is available.

Development of an HPLC Assay to Study the Effect of Endogenous and Exogenous Substances on Heat-Induced Aggregation of Human Serum Albumin