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A simple and flexible isocratic HPLC procedure was developed for the measurement of catecholamines and their abundant metabolites ( e.g. nor- metanephrine, metanephrine, methoxy-tyramine, vanillylmandelic acid, homovanillic acid) and 5-hydroxyindolacetic acid by ion-pair reversed phase chromatography on C18 columns, using a single mobile phase containing both sodium octane sulphonate and diethylamine as ion-pairing reagents.

clinical chemistry and laboratory medicine

Quantification of Urinary Catecholamines, their Abundant Metabolites, and 5-Hydroxyindoleacetic Acid by High Performance Liquid Chromatography and Electrochemical Detection, Using a Single Mobile Phase and Uniform Isocratic Conditions