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The concentrations of immunoglobulins IgG, IgM and IgA and their age dependence were determined in the serum of normal, untreated beagle dogs by a newly developed sandwich enzyme-linked-immuno-sorbent-assay (ELISA). A clear age-dependent increase between approximately 0.8 and 1.6 years of age was observed for the immunoglobulin IgA, whereas IgG and IgM showed only a slight tendency to an age-dependent increase. For immunotoxicological characterization of various compounds especially in long term studies, this IgA age-dependence has to be considered in the planning and interpretation of studies with beagle dogs.

clinical chemistry and laboratory medicine

Concentrations in Serum of IgG, IgM and IgA and Their Age-Dependence in Beagle Dogs as Determined by a Newly Developed Enzyme-Linked-Immuno-Sorbent-Assay (ELISA)