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A laser immunonephelometric procedure for the determination of human apolipoproteins A-I, B, and E in serum and lipoproteins was developed. Coefficients of variation were 2.3%-3.4% within-run, 4.7%-4.9% between-run. Serum apolipoprotein A-I values were similar in normal and hyperlipoproteinaemic subjects with a correlation coefficient of r = 0.585 to HDL-cholesterol levels. LDL-apolipoprotein B values were highly (r = 0.935), VLDL-apolipoprotein B values less strongly (r = 0.472) correlated to the corresponding cholesterol levels. Serum apolipoprotein E concentrations were distinctly elevated in Type III and not correlated to cholesterol values.

Determination of Human Apolipoproteins A-I, B, and E by Laser Nephelometry