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Physiochemical studies were carried out on the tricarbocyanine dye indocyanine green in biological fluids and organic solvents. The molar lineic absorbance epsilon of the compound was highest in organic solvents (methanol, 1.2-propanediol, dimethylformamide) and bile, but lowest in water and duodenal fluid. Indocyanine green remained stable in methanol and bile (t1/2 greater than 1 year) but was rapidly decomposed to a colourless derivative in duodenal fluid and distilled water (t1/2 3.6 days and 1.4 days, respectively). It was thermostable (120 degrees C) in methanol and 1.2-propanediol but thermolabile in water and dimethylformamide where the activation energy for the decomposition reaction was low. At ambient temperature (20 degrees C) indocyanine green was particularly labile at pH less than 5 and pH greater than 11. The rate of decay of indocyanine green in various solvents indicated that the rate limiting step in the decay process was either a first or zero order reaction.

Physicochemical Studies on Indocyanine Green: Molar Lineic Absorbance, pH Tolerance, Activation Energy and Rate of Decay in Various Solvents