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For the detection of total IgE in human sera different radioimmunoassay methods have been developed commercially, i.e. the RIST (radio-immunosorbent test) and the FRIST (= paper-radio-immunosurbent test) (l, 2). Since the FRIST is the most sensitive System, this kit is now generally used for the detection of total IgE (2). A similar radipimmunoassay has been developed for the detection of specific IgE (i.e. that part of the total IgE which is directed against a certain allergen). This kit is known as the RAST (= radio-allergosorbent test). Originally all these kits used radioactive compounds.

Comparison of Commercially Available RIA and ELISA Kits for the Determination of Total and Specific IgE in Human Sera