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A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase. Percentage recovery of added oxalate was 99 +/- 4 in serum, and 98 +/- 4 in urine (n - 10). Oxalate serum levels varied from 16.9 to 44.8 mumol/l. Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples. The detection limit is 5 mumol/l.

Direct Spectrophotometric Determination of Serum and Urinary Oxalate with Oxalate Oxidase