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Recently STORK and SCHMIDT (1) described an enzymatic method for the rapid determination of glucose in small blood samples. They used the well-known hexokinase reaction coupled with the glucose-6-phosphate dehydrogenase reaction to oxidize glucose to 6-phosphogluconate in the presence of NADP. Since the hemolyzed blood samples were introduced directly into the assay without prior precipitation of proteins, the whole procedure lasted only a few minutes from the capillary puncture until the result was available. This method seemed to be valid for the handling of a hyperor hypoglycaemic coma when single and rapid determinations are needed. The reliability of the method was therefore studied.

The Rapid, Enzymatic Determination of Glucose in Hemolysates