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A solution of glycollic acid in a mixture of benzyl alcohol and hexamethylphosphoric triamide is proposed to replace acetic acid in the glucose determination with 0-toluidine as applied directly to blood plasma. The new reagent is non-volatile, odourless, colourless, of low viscosity and stable for weeks at room temperature. It does not precipitate serum proteins. The method is particularly suited to discrete mechanization. Its sensitivity and overall reliability are higher than that of the acetic-acid method.

A New Medium for the Aldohexose- o -Toluidine Reaction: Direct Microdetermination of Blood Glucose