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Background:  Teicoplanin is a glycopeptide antibiotic used for the treatment of infections caused by Gram-positive bacteria. There is a good correlation between trough levels and clinical outcome, therefore therapeutic drug monitoring is recommended. Here we present a liquid chromatography-mass spectrometry (LC-MS) method with online extraction based on turbulent flow chromatography for the quantification of the five main components of teicoplanin, A2–1, A2–2, A2–3, A2–4, and A2–5.

Quantification of teicoplanin in plasma by LC-MS with online sample clean-up and comparison with QMS ® assay