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Background:  The relationship between fractional cholesterol esterification rate in plasma or serum high-density lipoprotein (HDL) (FER HDL ) and lipoprotein subfractions and other cardiovascular disease (CVD) risk factors has been demonstrated. However, the current method for measuring FER HDL  requires fresh serum samples and radioactive labeling of the samples, making it impractical for use in clinical laboratories. In this study, we developed a simple and precise HPLC method for the measurement of FER HDL . Correlations between FER HDL  and CVD risk factors were evaluated in 119 healthy volunteers.

clinical chemistry and laboratory medicine

A simple and precise method for direct measurement of fractional esterification rate of high density lipoprotein cholesterol by high performance liquid chromatography