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Background:  Several recent studies have shown some discrepancies between 25-hydroxyvitamin D [25(OH)D] assay methods, despite some improvement in the past few years. The accuracy of 25(OH)D assay methods is still a real challenge for clinical laboratories. The aim of this study was to assess the agreement between a large panel of routine assays and a two-dimensional liquid chromatography/tandem mass spectrometry (2D LC-MS/MS) method, selected as the reference method.

clinical chemistry and laboratory medicine

Agreement of seven 25-hydroxy vitamin D 3  immunoassays and three high performance liquid chromatography methods with liquid chromatography tandem mass spectrometry