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Media components were optimized using two-step statistical design of experiments for enhancing endoglucanase/ carboxymethyl cellulase (CMCase) production by  Trichoderma reesei  RUT C30. A Placket-Burman design identified cellulose concentration and pH as the most significant variables, which influenced the CMCase activity. Central composite design was employed to optimize these selected parameters. The optimal activity was obtained at cellulose concentration 19.7 g/L and pH of 7.2. Under the optimized conditions, CMCase activity was 83.63 ± 1.86 IU/mL and filter paper activity was 2.58 ± 0.2 filter paper units per mL. Enzyme productivity was higher compared to previous reports. The enzyme produced from  T. reesei  was concentrated and was evaluated for deinking of printed paper, which demonstrated the suitability of the enzyme for this application.

Production of endoglucanase from Trichoderma reesei RUT C30 and its application in deinking of printed office waste paper