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Sheep play an important role in agricultural production and people’s lives, and fecundity is one of the most important economic traits of sheep for sheep breeders. The Booroola fecundity ( FecB ) gene has a certain correlation with litter size in sheep. Therefore, this study aims to detect  FecB  expression quickly, accurately and visually. Here, we used the nucleic acid dye SYBR Green I to detect  FecB  with the amplification refractory mutation system (ARMS), which can efficiently, rapidly, economically and visually detect  FecB  expression in sheep. After ARMS polymerase chain reaction (PCR), SYBR Green I was directly added to the ARMS products, and whether the sheep carried  FecB  was judged by directly observing the color change in the PCR tube. Homozygous (BB) or heterozygous (B+) samples with  FecB  mutation were bright green, while wild type (++) samples without  FecB  were orange yellow. This study suggested that this method has 100% accuracy and 0.5 ng/µL sensitivity. To our knowledge, this is the first report that shows the integration of the ARMS with SYBR Green I to detect  FecB  expression in sheep visually.

Rapid visual detection of FecB gene expression in sheep