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The regulation of triacylglycerol synthesis by various long-chain fatty acids was studied using primary cultures of hepatocytes. The activity of diacylglycerol acyltransferase was measured after 72-h incubation with various fatty acids in equimolar concentrations (0.5 mM): oleate, linoleate, linolenate, palmitate, stearate and arachidonate increased enzyme activity to 191%, 161%, 148%, 124%, 116% and 114%, respectively, compared to controls. Cellular triacylglycerol levels showed the same changes, suggesting a regulatory function of diacylglycerol acyltransferase in triacylglycerol synthesis. Elaidate lowered the enzyme activity by 57%. The addition of different fatty acids to the incubation medium did not influence the distribution of enzyme activity in the 30 000 x g pellet which was routinely discarded and the microsomal fraction (105 000 x g), where the enzyme was predominantly localized (91%). The release of lactate dehydrogenase into the culture medium of hepatocyte monolayers as well as ketone body production was not affected by any of the fatty acids.

Influence of Fatty Acids on Microsomal Diacylglycerol Acyltransferase Activity in Primary Cultures of Hepatocytes