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The microsomal 3-hydroxysteroid dehydrogenases were solubilized with lubrol, a non-ionic detergent. A 3alpha-hydroxysteroid dehydrogenase is purified about 100-fold by double affinity chromatography on 5alpha-dihydrotestosterone-Sepharose. This enzyme can use both NADH and NADPH as coenzymes.

Solubilisierung und Anreicherung einer 3α-Hydroxysteroid-Dehydrogenase aus Rattenleber-Mikrosomen