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Purpose: Our goal was to develop two-tier strategy based on in house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14

acta medica martiniana

Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective &amp; Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions