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To provide a means for studying certain immunological phenomena in mouse splenic tissue in vitro we have recently developed a system for maintaining mouse spleens in organ culture. Although previous attempts by some investigators to culture spleen tissue in organ culture have produced encouraging results (Globerson and Auerbach 1965, Globerson and Auerbach 1966, Holtermann and Nordin 1968, Klinman 1972, Pinkel 1963), others have reported difficulty in getting spleen organ cultures to survive in vitro, even when methods are used that enjoy considerable success with other types of tissue (Trowell 1959). The purpose of the present report is to describe the organ culture system we have found to be satisfactory and to define some of the properties of the cultured spleen fragments and whole spleens. Atten tion has been paid to possible reasons for the previously reported variability in the survival of splenic explants; in particular the influence of the size of the explant has been assessed and found to be of critical importance.

Maintenance of Mouse Spleen in Organ Culture and Assessment of Certain Functional Capabilities