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A solid phase radioimmunoassay for the detection of anti-human thyroglobulin antibodies using 125I-protein A has been developed and its clinical use was evaluated in 20 patients with Graves' disease and 20 patients with Hashimoto's thyroiditis. After coating polystylene tubes with human thyroglobuline (102mg/m1), 500 of sera were incubated for 2 hours at 25°C followed by the addition of 1251-protein A. After incubation, the tubes were washed extensively to remove free125I-protein A, and radioactivities of the bound 125 'protein A were counted. The results of this solid phase radioimmunoassay were compared with those obtained by two other commercially available kits: Thyroid test and Thyroglobulin antibody kit (1251HTg method). Based on the results of the 125I-HTg and 125 I-protein A method, obtained from the sera of 26 healthy subjects, normal range of P/N1 of the 125 I.-HTg method (P: patient, N1: anti-HTg antibody free serum) and P/N2 of the 125I-protein A method (P: patient, N2: mean of healthy subjects) were obtained. P/N1 higher than 0.92 and P/N2

A Solid Phase Radioimmunoassay using <SUP>125</SUP>I-protein A for the Detection of Anti-Human Thyroglobulin Antibodies