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Summarizing the results obtained from our experiments'), ovulatory release of LH with a peak around 7 p.m. of proestrus depends on the foregoing increase in estrogen secretion starting in the afternoon of diestrus II, and a certain amount of estrogen secreted during. the period from 4 a.m. to 7 a.m. of proestrus is definitely essential for occurrence of LH release under our breeding conditions. Estrogen secretion from the ovary with mature follicles is completely under the control of the pituitary, and it is not automatically derived from the secretory function of developing tollicles bui sustained and stimulated by both LH and FSH. A drastic fall of ovarian estrogen secretion immediately after the maximal elevation of plasma LH is not due to the deficiency of pituitary factor, which is responsible for estrogen secretion, but due to the depletionn of ovarian reactivity to the factor. Preovulatory increase in progesterone secretion is entirely under the control of the

Role of Ovarian Steroids on the Ovulatory Release of Luteinizing Hormone in the Rat