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Pregnant Mare's Serum Gonadotrophin (PMS) and Human Chorionic Gonadotrophin (HCG) were used in 110 anovulatory women who were suffering from various types of ovarian insufficiency. As a result, in 65 out of 110 patients (59.1%) ovulation was induced. Clinically, it was clear that larger amounts of PMS were needed in the second course than in the first course. This evidence is presumably due to the inhibition of PMS activity by anti-PMS appeared in the blood. It has been discussed that PMS might act as an antigen in the human body because it is a heteroproteohormone. The follwoing experiments were performed immunologically: 5 c.c. of blood were obtained from sixteen patients selected randomly out of the patients treated with gonadotrophin. The sera were incubated at 56°C for 30 min. Anti-PMS titers were determined by using the haemagglutination reaction before, through, and after the treatment. The anti-PMS titers could not be detected before the treatment in all cases, and with some individual differences, at least 1:128 of the titers were observed in all cases and continued for about 20 weeks. The blood obtained from eleven patients treated repeatedly 8-30 weeks after the first therapy was tested with the same method. Generally, titers increased rapidly, maintained a high level and continued for a longer period than the term after the first course. In order to investigate whether or not the anti-PMS which was detected by haemag-

Studies on Anti-gonadotrophin in the Serum of Patients Treated with the Pregnant Mare's Serum Gonadotrophin