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Cortolone, Cortol and 11-deoxy-Cortol have been not determined as 17-OHCS or Porter-Silber chromogen, although they form one of the main groups of urinary metabolites of glucocorticoids. In this study, a new simple method for fractional determination of these three cortol compounds in urine was established. The procedure was carried out as follows : 1) Hydrolysis with 8-glucuronidase. 2) Extraction with ethyl acetate. 3) Separation of cortol compounds by Florisil column chromatography. 4) Oxidation with periodic acid, 11-deoxy-cortol was converted into etiocholanolone, cortol into 11-ie-hydroxy-etiocholanolone, cortolone into 11-keto-etiocholanolone. 5) Fractionation of three converted steroids by 6% water alumina column chromatography. 6) Colorimetry as Zimmermann chromogen. Total recovery rate was 66.2 =L.2.0%. Normal mean excretion values were as follows : Subjects Cases 11-deoxy-Cortol Cortolone Cortol Sum Total Normal Prolif. 20 178.8•}98.2 1120.6•}241.2 734.9•}198.1 2034.3•}145.8 Females Secret. 20 207.6•}58.9 975.2•}132.4 462.8•}169.6 1645.6•}120.3 Normal Males 20 264.9•}49.2 1514.9•}227.3 757.5•}181.2 2537.3•}152.7 Castrated Women 20 199.6•}85.5 876.5•}288.1 560.4•}149.9 1636.5•}141.1 μg pcr day±C.L. (pp. 653-,-656)

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Study on a New Simple Method for the Determination of Cortol (5β-pregnan-3α, 11β, 17α, 20, 21-pentol), Cortolone (3α, 17α, 20, 21-tetrahydroxy-5β-pregnan-11-one) and 11-deoxy-Cortol (5β-pregnan-3α, 17α, 20, 21-tentol) and Their Urinary Excretion Values in Normal Subjects.