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| A study was conducted to measure the effect of melatonin (MT) on motility and velocity parameters of mithun semen by Computer Assisted Sperm Analyser (CASA) in different seasons. Total numbers of 80 ejaculates (20 ejaculates in each season) were collected twice a week from mithun bulls and were split into five equal aliquots, diluted with the Tris Egg Yolk Citrate (TEYC) extender. Group 1: semen without additives (control), group 2 to group 6: semen was diluted with 1, 2, 3, 4 and 5 mM of melatonin, respectively. CASA parameter such as Forward Progressive Motility (FPM), Total Motility (TM), Curvilinear Velocity (VCL), Straight Line Velocity (VSL), Average Path Velocity (VAP), Linearity (LIN), Straightness (STR), Amplitude of Lateral Head displacement (ALH) and Beat / Cross Frequency (BCF) were measured after 12 hrs of incubation at 5oC. Blood samples were collected in different seasons to estimate the melatonin. The results revealed that these parameters were varied significantly (p<0.05) among the different experimental groups and among the seasons. Further, MT at 3 mM has significant improvement than other treatment groups stored in invitro at different seasons. Additionally, spring season has highest value followed by winter and autumn season, whereas lower values were in summer season. Melatonin concentration was higher in winter season and lowest was in summer season. The result of the study indicated that the melatonin protects CASA parameters, varied in different seasons. It was concluded that ejaculates from spring and winter season has significantly higher CASA parameters in mithun.

Casa Parameters of Mithun Semen Treated with Melatonin