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Chromosomal aberrations play an important role as prognostic factors in chronic lymphocytic leukemia (CLL). These aberrations are mostly detected by fluorescent in situ hybridization (FISH), as chromosomal banding analysis has been scarce due to low proliferative activity of malignant B-lymphocytes in vitro. In 2006, a new method using stimulation with IL-2 and CpG oligonucleotide DSP30 for metaphase generation in CLL was published [1]. The objective of our study was to verify the efficacy of stimulation and to evaluate if the method is suitable for routine diagnostics.

klinická onkologie

Chromosome Banding Analysis of Peripheral Blood Lymphocytes Stimulated with IL-2 and CpG Oligonucleotide DSP30 in Patients with Chronic Lymphocytic Leukemia