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Herbal industry in Indonesia is progressing very rapidly. Increasing number of herbal medicine industries lead to an increase of the waste which are normally processed in anaerobic ponds by using chemical and biological process. However this process are not economical feasible and therefore an alternative method by using natural resource is required. Phytoremediation is an environmental friendly method to reduce contaminant using aquatic plant. This method uses water plant to reduce COD and nutrients content in the waste. Since the waste still high content of nutrient, therefore it is potential for medium growth of algae Spirulina. This study was aimed to evaluate the use of various plant species (water hyacinth and lotus) in decreasing contaminant and to determine optimal nutrient composition of the growth media. The phytoremediation was performed in 3-8 days and height of liquid in the tank was maintained constant at 5 cm. The effluent of first phytoremediation was transferred to second stage for cultivation of Spirulina with 15 days of cultivation time. The external nutrients were added each 2 days and the concentration of biomass was measured for its optical density. Spirulina grow well in herbal medicine waste that has been phytoremediation with lotus for 3 days and had a CNP ratio amounted to 57.790 : 9.281 : 1 with a growth rate of 0.271/day. Industri obat di Indonesia tumbuh sangat cepat. Pertumbuhan ini mengakibatkan meningkatnya limbah yang umumnya dilakukan dalam kolam anaerobik dengan menggunakan proses kimia dan biologi. Namun demikian, proses tersebut belum menunjukkan hasil ekonomis sehingga diperlukan metode lain yang relatif aman dan ekonomis. Salah satu cara yang dapat digunakan adalah phytoremediasi mengguanakn tanaman air untuk mengurangi kandungan COD dalam limbah dan nutrient. Dikarenakan limbah masih mengandung nutrient yang cukup banyak, maka limbah tersebut juga sangat potensial untuk digunakan sebagai medium mikroalga. Penelitian ini bertujuan untuk mengevaluasi penggunaan tanaman air (enceng gondok dan teratai) untuk mereduksi kontaminan dalam limbah obat jamu. Phytoremediasi dilakukan selama 4-8 hari dan tinggi cairan dalam reaktor dijaga pada 5 cm. Keluaran dari phytoremediasi pertama menggunakan tanaman air digunakan sebagai medium di phytoremediasi menggunakan mikroalga Spirulina. Untuk mendapakan pertumbuhan yang optimum, maka ditambahakan juga nutrient dan menunjukkan bahwa Spirulina tumbuh dengan sangat baik dalam medium ini. Pertumbuhan terbaik diperoleh dari phytoremediasi menggunakan teratai selama 3 hari dan kecepatan pertumbuhan 0,271/hari dengan perbandingan C:N:P = 57,790:9,28:1.

APPLICATION OF PHYTOREMEDIATION FOR HERBAL MEDICINE WASTE AND ITS UTILIZATION FOR PROTEIN PRODUCTION