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Bovine babesiosis is an enzootic disease caused by Apicomplexa protozoa from Babesia genus. In Argentine, the etiological agents are Babesia bovis and Babesia bigemina, both transmitted by Rhipicephalus microplus. Studying the biology and the intraespecific genetic variations of B. bigemina from Argentinean and other region strains has become an easy task after the development of continuous in vitro multiplication for these parasites. The studies were based on the use of strains and clones which patogenicity level was established in vivo. Clones were obtained by the limiting dilution technique and showed similar in vitro multiplication efficiency and the same phenotype as their parental strains. 18S rRNA, rap1c, gp45, ITS genes and repeat sequences were compared. The 18S rRNA gene allowed strains differentiation by using punctual mutations visualized in the secondary structure of E23–1 helix. Individually, rap–1c gene was used to identify strains associated to patogenicity, confirmed with HRM technique. Differences existing between not geographically related strains were identified by using 18S rRNA and repeat sequences. Finally, the population selection after the strains adaptation to the in vitro growth was confirmed through the simultaneous analysis of 18S rRNA and rap1c sequences from clones. As a conclusion, clones and molecular markers selected allowed to establish the diversity among different Argentinean regions and among these and foreign strains. Differences between the virulence of the strains helped us to gain knowledge of the patogenicity from B. bigemina natural isolates.

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Estudios funcionales de factores de transcripción vegetales de la familia TCP. Análisis de su participación en la regulación del crecimiento y la proliferación celular, y en la coordinación de la biogénesis mitocondrial