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(Received: October 27, 2015; Revised: December 16, 2015; Accepted: December 24, 2015)Abstract : Exercise is one of the most common stressors in horses. Although various physiological parameters suchas cortisol respond to exercise, there is no reliable parameter for the measurement of exercise-induced stress insport horses. This study was performed to discover a new biomarker with high sensitivity for exercise-induced stress.The expression of fos mRNA was increased more than 10-fold in horse blood samples collected after an hour ofexercise, as compared with before the exercise. The plasma cortisol levels were also increased after the exercise,but only by about two-fold. The fos mRNA levels were well-correlated with plasma cortisol concentrations. Thesefindings suggest that fos mRNA expression in blood may be useful for the measurement of exercise-induced stressin horses.Keywords : biomarker, exercise, fos, horse, stress

A novel biomarker of exercise-induced stress in horses