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Haemoparasites belonging to filum Apicomplexa deserve attention because can affect the conservation of mammals’s biodiversity. This work aimed to avalue the occurencce of Piroplasmida agents (Cytauxzoon, Theileria e Babesia) e Hepatozoon spp. using Convencional Microscopic Exam (CME) and Polymerase Chain Reaction (PCR) and indetify ticks on wild mammals belonging to LAPAS-UFU, CETAS-GO/IBAMA e CETASBH/IBAMA. On total, this work obtained 152 total blood samples that were submited to PCR for Hepatozoon spp. with primers HepF300/Hep900, Hep2F/Hep2R e HAM-1/HPFR2, and for Piroplasmida agents, PCR was done using primers BAB33-47/ BAB432-439, PIRO 1F/PIRO 5R. To exam slides on optic microscope, was used peripheral samples of 123 mammals. There wasn’t observed gamonts of Hepatozoon spp. on blood slides, but on PCR was observed prevalence of 5,2% (eight positive mammals). From these, was obtained four new haplotypes of Hepatozoon spp. like similar sequence of Hepatozoon canis in manedwolf (Chrysocyon brachyurus) and hoary fox (Lycalopex vetulus) and similar sequence of Hepatozoon felis in tayra (Eira barbara) and puma (Puma concolor). For Piroplasmida agents, the CME and PCR results were 34,9% (43 positive mammlas) and 26,3% (40 positive mammals), respectively. There was obtained three new haplotypes of Piroplasmida agents, being similar sequences of Theileria cervi in brown brocket deer (Mazama gouazoubira), similar sequence of Theileria equi in brazilian tapir (Tapirus terrestris) and similar sequence of Cytauxzoon felis in ocelot (Leopardus pardalis). There was identified 432 specimnes of ticks on 30 mammals. The species were Amblyomma nodosum, A. parvum, A. ovale, A. sculptum, A. longirostre, A. dubidatum, Rhipicephalus microplus e R. sanguineus. Besides this results, other haemoparasites were observed on optic microscope, like Ehrlichia spp. in 23 mammals (18,6%) and microfilaria in two mammals of the species Coendou prehensilis (brazilian porcupine). The information gathered by this work confirms the abundance of haemoparasites that the Brazilian mammals can harbor. In addition, some species of ticks identified were determinant to infer possible ways of transmitting hemoparasites identified in mammals. The result obtained by the use of molecular diagnostic techniques (PCR and sequencement) was more accurate than the CME for identification of hemoparasites on wild mammals of this work. Palavras-chave: Haemoparasites. Wild mammal. Optic microscope. Polymerase Chain Reaction. Apicomplexa. Ixodidae. LISTA DE ABREVIATURAS C: Cativeiro CEUA: Comitê de Ética Animal CETAS: Centro de Triagem de Animais Silvestres EMC: Exame Microscópico Convencional FAMEV: Faculdade de Medicina Veterinária IBAMA: Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis ICBIM: Instituto de Ciências Biomédicas LAPAS: Laboratório de Ensino e Pesquisa em Animais Silvestres n: Número de amostras pb: Pares de bases PCR: Polymerase Chain Reaction SISBIO: Sistema de Autorização e Informação em Biodiversidade SMF: Sistema Mononuclear Fagocítico UFU: Universidade Federal de Uberlândia USP: Universidade de São Paulo V: Voltagem VL: Vida livre %: Porcentagem/Por cento μm: micrômetro

Ocorrência de Hepatozoon spp., Piroplasmas, Ehrlichia spp. e filarídeos em mamíferos silvestres de Centros de Triagem de Minas Gerais e Goiás