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Ethyl palmitate is the ester derivatives of palmitic acid which is a saturated fatty acid. Chemical formula of this compound is C18H36O2. It is a colorless solid with a wax-like odor. Esters are important organic compounds with increasing number of commercial applications [1]. Ethyl palmitate are infrequent found in the nature, except the base material synthesized from saturated fatty acids, such as palmitic acid. Palmitic acid contained in palm oil (39.2-45.8 %) [2], corn oil 8.0-12.0 %, soybean oil 7-10 %, canola oil 3.0-7.0 %, 4.1-17.3 %, sun flower, 2.0-6.0 %, linseed 4.0-7.0 % [3], coconut 4.6-20.0 % [4]. Transesterification reaction of oils which contained palmitic acid and ethanol will produce ethyl palmitate. Ethyl palmitate can be used as a raw material in the manufacture of cosmetics, detergents, additives at the textile and paper [5], flavors and pharmaceuticals and hydraulic fluids [6]. Ethyl palmitate is used as a hair and skin-conditioning agent as well. Ethyl palmitate in commercial applications has been hampered due to the low amounts that can be recovered from natural sources [7,8]. Therefore, most of the available esters are produced by chemical or enzymatic synthesis. Chemical transesterification method leads to high energy consumption and degradation of ester produced. Meanwhile, The biotechnological production of ethyl palmitate using lipases has received particular attention due to the mild reaction conditions Four Factor Surface Response Optimization of Ethyl Palmitate from Terminalia cattapa L. Kernel Oil