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For a transparent well with a known volume capacity, changes in fluid levelresult in predictable changes in magnification of an overhead light source. Fora given well size and fluid, the relationship between volume and magnificationcan be calculated if the fluid's index of refraction is known or in a naivefashion with a calibration procedure. Light source magnification can bemeasured through a camera and processed using computer vision contour analysiswith OpenCV. This principle was applied in the design of a 3D printable sensingdevice using a raspberry pi zero and a camera

A computer vision based optical method for measuring fluid level in cell culture plates