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Sclerotinia sclerotiorum  and  Botrytis cinerea  are notorious plant pathogenic fungi with an extensive host range including Brassica crops. Glucosinolates (GSLs) are an important group of secondary metabolites characteristic of the Brassicales order, whose degradation products are proving to be increasingly important in plant protection. Enhancing the defense effect of GSL and their associated degradation products is an attractive strategy to strengthen the resistance of plants by transgenic approaches. We generated the lines of  Brassica napus  with three biosynthesis genes involved in GSL metabolic pathway ( BnMAM1 ,  BnCYP83A1  and  BnUGT74B1 ), respectively. We then measured the foliar GSLs of each transgenic lines and inoculated them with  S .  sclerotiorum  and  B .  cinerea . Compared with the wild type control, over-expressing  BnUGT74B1  in  B .  napus  increased the aliphatic and indolic GSL levels by 1.7 and 1.5 folds in leaves respectively; while over-expressing  BnMAM1  or  BnCYP83A1  resulted in an approximate 1.5-fold higher only in the aliphatic GSL level in leaves. The results of plant inoculation demonstrated that  BnUGT74B1 -overexpressing lines showed less severe disease symptoms and tissue damage compared with the wild type control, but  BnMAM1  or  BnCYP83A1 -overexpressing lines showed no significant difference in comparison to the controls. These results suggest that the resistance to  S .  sclerotiorum  and  B .  cinerea  in  B .  napus  could be enhanced through tailoring the GSL profiles by transgenic approaches or molecular breeding, which provides useful information to assist plant breeders to design improved breeding strategies.

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Overexpression of Three Glucosinolate Biosynthesis Genes in Brassica napus Identifies Enhanced Resistance to Sclerotinia sclerotiorum and Botrytis cinerea