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Gross chromosomal rearrangements have the potential to be evolutionarilyadvantageous to an adapting organism. The generation of a hybrid speciesincreases opportunity for recombination by bringing together two homologousgenomes. We sought to define the location of genomic rearrangements in threestrains of Saccharomyces pastorianus, a natural lager-brewing yeast hybrid ofSaccharomyces cerevisiae and Saccharomyces eubayanus, using whole genomeshotgun sequencing. Each strain of S. pastorianus has lost species-specificportions of its genome and has undergone extensive recombination, producingchimeric chromosomes. We predicted 30 breakpoints that we confirmed at thesingle nucleotide level by designing species-specific primers that flank eachbreakpoint, and then sequencing the PCR product. These rearrangements are theresult of recombination between areas of homology between the two subgenomes,rather than repetitive elements such as transposons or tRNAs. Interestingly,28/30 S. cerevisiae- S. eubayanus recombination breakpoints are located withingenic regions, generating chimeric genes. Furthermore we show evidence for thereuse of two breakpoints, located in HSP82 and KEM1, in strains of proposedindependent origin.

plos one

Sequencing and characterisation of rearrangements in three S.  pastorianus strains reveals the presence of chimeric genes and gives evidence  of breakpoint reuse